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Quiz time/ 03
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Quiz time/ 03
MCQ'S - gel electrophoresis
1. In an SDS-PAGE
A. proteins are denatured by the SDS
B. proteins have the same charge-to-mass ratio
C. smaller proteins migrate more rapidly through the gel
D. all of the above
2. Electrophoresis is used to
A Separate DNA fragments.
B Identify DNA fragments.
C Purify DNA fragments.
D Both A and B
E Both A and C
3. The rate at which DNA migrates through the gel is determined by:
A Molecular size of the DNA and the agarose gel concentration.
B Conformation of DNA and the applied voltage.
C The length of the agarose gel and the negativity of the DNA.
D Both A and B
E Both A and
4. Why do scientists load DNA of known sizes into the agarose gel?
A It makes it easier to determine sizes of unknowns using comparison techniques.
B To fill in all the slots on the gel so you can run it.
C To practice loading the DNA before you get to the important DNA.
D So you know how long the gel needs to run.
5. How can one tell if their gel electrophoresis is running properly?
A It bubbles.
B You can see the methyl blue move from the well into the gel.
C The DNA runs to red
D Both B and C
E All three choices A, B and C
1. In an SDS-PAGE
A. proteins are denatured by the SDS
B. proteins have the same charge-to-mass ratio
C. smaller proteins migrate more rapidly through the gel
D. all of the above
2. Electrophoresis is used to
A Separate DNA fragments.
B Identify DNA fragments.
C Purify DNA fragments.
D Both A and B
E Both A and C
3. The rate at which DNA migrates through the gel is determined by:
A Molecular size of the DNA and the agarose gel concentration.
B Conformation of DNA and the applied voltage.
C The length of the agarose gel and the negativity of the DNA.
D Both A and B
E Both A and
4. Why do scientists load DNA of known sizes into the agarose gel?
A It makes it easier to determine sizes of unknowns using comparison techniques.
B To fill in all the slots on the gel so you can run it.
C To practice loading the DNA before you get to the important DNA.
D So you know how long the gel needs to run.
5. How can one tell if their gel electrophoresis is running properly?
A It bubbles.
B You can see the methyl blue move from the well into the gel.
C The DNA runs to red
D Both B and C
E All three choices A, B and C
Re: Quiz time/ 03
1) D. all of the above
2) E Both A and C
3) D. Both A and B
4) A. It makes it easier to determine sizes of unknowns using comparison techniques.
5) E. All three choices A, B and C
2) E Both A and C
3) D. Both A and B
4) A. It makes it easier to determine sizes of unknowns using comparison techniques.
5) E. All three choices A, B and C
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